Репозиторий Университета

© 2020, Springer Science+Business Media, LLC, part of Springer Nature. Label-free characterization of cell subpopulations is a very promising biomedical approach. Nowadays, there are several label-free methods based on different physical properties such as size, density, stiffness, etc. allowing the characterization of biological objects. However, fluorescence properties are the most suitable feature for the label-free study of tissue and cells. Understanding the autofluorescence level peculiarities of normal and pathological / live and dead cells can become a helpful tool for cells’ metabolic activity, viability evaluation, and diagnostics of a number of diseases. In this study, we applied a series of mouse cell lines (RAW 264.7 - macrophages, L929 - fibroblasts, C2C12 – myoblasts, and B16-F10 – melanoma) to compare cell autofluorescence of live and dead cells under 488 nm laser excitation and found the difference between their autofluorescence depending on a cell state and type.

© 2020, Ecozone, OAIMDD. All rights reserved. The article presents the historical stages of the formation and development of the ideas of Deontology and medical ethics and highlights the world community‟s interest in relevant problems of Bioethics. The principles of ethical-deontological relations are shown on the example of priest-doctors‟ difficult but at the same time vivid life path. The priests presented in the article are great personalities in world history.

© 2020, Ecozone, OAIMDD. All rights reserved. The article presents the historical stages of the formation and development of the ideas of Deontology and medical ethics and highlights the world community‟s interest in relevant problems of Bioethics. The principles of ethical-deontological relations are shown on the example of priest-doctors‟ difficult but at the same time vivid life path. The priests presented in the article are great personalities in world history.

© 2020, The Author(s). Objectives: To assess the trueness of a solid index (SI) in the full-arch (FA) implant impression, and to compare it with that of two intraoral scanners (IOSs). A type-IV gypsum model of a completely edentulous patient with 8 implant scanbodies (SBs) was scanned with a desktop scanner (7Series®) to obtain a reference virtual model (RVM), and with two IOSs (CS 3700® and Emerald S®). Five scans were taken with each IOS. Based on the RVM, an SI (custom tray consisting of hollow cylinders connected by a bar) was fabricated and used to capture a physical impression of the model; from this, a second gypsum model was derived and scanned with a desktop scanner (D15®). The SI-derived and the IOSs-derived models were superimposed onto the RVM, to evaluate trueness. Results: The overall mean trueness was 29 μm (± 26) for the SI-derived model, versus 42.4 μm (± 14.7) for CS 3700® and 52.2 μm (± 4.6) for Emerald S®. Despite its limitations (in vitro design, a limited number of models evaluated, RVM captured with a desktop scanner) this study supports the use of SI for FA implant impressions. Further studies are needed to confirm this evidence.

© 2020, Ecozone, OAIMDD. All rights reserved. The article presents the historical stages of the formation and development of the ideas of Deontology and medical ethics and highlights the world community‟s interest in relevant problems of Bioethics. The principles of ethical-deontological relations are shown on the example of priest-doctors‟ difficult but at the same time vivid life path. The priests presented in the article are great personalities in world history.

© 2020, Ecozone, OAIMDD. All rights reserved. The article presents the historical stages of the formation and development of the ideas of Deontology and medical ethics and highlights the world community‟s interest in relevant problems of Bioethics. The principles of ethical-deontological relations are shown on the example of priest-doctors‟ difficult but at the same time vivid life path. The priests presented in the article are great personalities in world history.

© 2020, Ecozone, OAIMDD. All rights reserved. The article presents the historical stages of the formation and development of the ideas of Deontology and medical ethics and highlights the world community‟s interest in relevant problems of Bioethics. The principles of ethical-deontological relations are shown on the example of priest-doctors‟ difficult but at the same time vivid life path. The priests presented in the article are great personalities in world history.

© 2020, Ecozone, OAIMDD. All rights reserved. The article presents the historical stages of the formation and development of the ideas of Deontology and medical ethics and highlights the world community‟s interest in relevant problems of Bioethics. The principles of ethical-deontological relations are shown on the example of priest-doctors‟ difficult but at the same time vivid life path. The priests presented in the article are great personalities in world history.

© 2020, Ecozone, OAIMDD. All rights reserved. The article presents the historical stages of the formation and development of the ideas of Deontology and medical ethics and highlights the world community‟s interest in relevant problems of Bioethics. The principles of ethical-deontological relations are shown on the example of priest-doctors‟ difficult but at the same time vivid life path. The priests presented in the article are great personalities in world history.

© 2020 John Wiley  &  Sons, Ltd. GMDP (glucosoaminyl-muramyl-dipeptide), a synthetic analog of the peptidoglycan fragment of the bacterial cell wall, is an active component of the immunomodulatory drug Licopid. But the pharmacokinetic parameters of GMDP in humans after oral administration have not been investigated yet. The present study aimed at developing and validating a sensitive LC–MS/MS method for the analysis of GMDP in human plasma. The sample was prepared by solid-phase extraction using Strata-X 33 μm polymeric reversed-phase 60 mg/3 mL cartridges Phenomenex (Torrance, CA, USA). The analytes were separated using an Acquity UPLC BEN C18 column, 1.7 μm 2.1 × 50 mm Waters (Milford, USA). GMDP and internal standard growth hormone releasing peptide-2 (pralmorelin) were ionized in positive electrospray ionization mode and detected in multiple reaction monitoring mode. The developed method was validated within a linear range of 50–3000 pg/mL for GMDP. Accuracy for all analytes, given as the deviation between the nominal and measured concentration and assay variability, ranged from 1.61 to 3.02% and from 0.89 to 1.79%, respectively, for both within- and between-run variabilities. The developed and validated HPLC–MS/MS method was successfully used to obtain the plasma pharmacokinetic profiles of GMDP distribution in human plasma.

© 2020, Ecozone, OAIMDD. All rights reserved. The article presents the historical stages of the formation and development of the ideas of Deontology and medical ethics and highlights the world community‟s interest in relevant problems of Bioethics. The principles of ethical-deontological relations are shown on the example of priest-doctors‟ difficult but at the same time vivid life path. The priests presented in the article are great personalities in world history.

© 2020, Ecozone, OAIMDD. All rights reserved. The article presents the historical stages of the formation and development of the ideas of Deontology and medical ethics and highlights the world community‟s interest in relevant problems of Bioethics. The principles of ethical-deontological relations are shown on the example of priest-doctors‟ difficult but at the same time vivid life path. The priests presented in the article are great personalities in world history.

© 2020, Ecozone, OAIMDD. All rights reserved. The article presents the historical stages of the formation and development of the ideas of Deontology and medical ethics and highlights the world community‟s interest in relevant problems of Bioethics. The principles of ethical-deontological relations are shown on the example of priest-doctors‟ difficult but at the same time vivid life path. The priests presented in the article are great personalities in world history.

© 2020, Ecozone, OAIMDD. All rights reserved. The article presents the historical stages of the formation and development of the ideas of Deontology and medical ethics and highlights the world community‟s interest in relevant problems of Bioethics. The principles of ethical-deontological relations are shown on the example of priest-doctors‟ difficult but at the same time vivid life path. The priests presented in the article are great personalities in world history.

© 2020, Ecozone, OAIMDD. All rights reserved. The article presents the historical stages of the formation and development of the ideas of Deontology and medical ethics and highlights the world community‟s interest in relevant problems of Bioethics. The principles of ethical-deontological relations are shown on the example of priest-doctors‟ difficult but at the same time vivid life path. The priests presented in the article are great personalities in world history.

© 2020, Ecozone, OAIMDD. All rights reserved. The article presents the historical stages of the formation and development of the ideas of Deontology and medical ethics and highlights the world community‟s interest in relevant problems of Bioethics. The principles of ethical-deontological relations are shown on the example of priest-doctors‟ difficult but at the same time vivid life path. The priests presented in the article are great personalities in world history.

© 2020, Ecozone, OAIMDD. All rights reserved. The article presents the historical stages of the formation and development of the ideas of Deontology and medical ethics and highlights the world community‟s interest in relevant problems of Bioethics. The principles of ethical-deontological relations are shown on the example of priest-doctors‟ difficult but at the same time vivid life path. The priests presented in the article are great personalities in world history.

© 2020, Ecozone, OAIMDD. All rights reserved. The article presents the historical stages of the formation and development of the ideas of Deontology and medical ethics and highlights the world community‟s interest in relevant problems of Bioethics. The principles of ethical-deontological relations are shown on the example of priest-doctors‟ difficult but at the same time vivid life path. The priests presented in the article are great personalities in world history.

© 2020, Ecozone, OAIMDD. All rights reserved. The article presents the historical stages of the formation and development of the ideas of Deontology and medical ethics and highlights the world community‟s interest in relevant problems of Bioethics. The principles of ethical-deontological relations are shown on the example of priest-doctors‟ difficult but at the same time vivid life path. The priests presented in the article are great personalities in world history.

© 2020, Ecozone, OAIMDD. All rights reserved. The article presents the historical stages of the formation and development of the ideas of Deontology and medical ethics and highlights the world community‟s interest in relevant problems of Bioethics. The principles of ethical-deontological relations are shown on the example of priest-doctors‟ difficult but at the same time vivid life path. The priests presented in the article are great personalities in world history.